Electrochemical Magnetoimmunosensing Approach for the Sensitive Detection of H9N2 Avian Influenza Virus Particles
Identifieur interne : 001B06 ( Main/Exploration ); précédent : 001B05; suivant : 001B07Electrochemical Magnetoimmunosensing Approach for the Sensitive Detection of H9N2 Avian Influenza Virus Particles
Auteurs : Chuan-Hua Zhou [République populaire de Chine] ; Yun Shu [République populaire de Chine] ; Zheng-Yuan Hong [République populaire de Chine] ; Dai-Wen Pang [République populaire de Chine] ; Zhi-Ling Zhang [République populaire de Chine]Source :
- Chemistry – An Asian Journal [ 1861-4728 ] ; 2013-09.
English descriptors
- Teeft :
- Anal, Bioelectron, Biosens, Catalytic reaction, Catalytic signal, Centrifuge tube, Chem, Chicken dung, Complex samples, Cyclic voltammograms, Detection solution, Different concentrations, Effective immunomagnetic separation, Electrochemical, Electrochemical detection, Electrochemical immunoassay, Electrochemical magnetoimmunosensor, Electrochemical signal, Electrochemical signals, Electrode, Error bars, First step, Glass slide, Glass slides, Gmbh, Good reproducibility, High sensitivity, Higher reproducibility, Homemade magneto, Immunoassay, Immunological reaction, Immunomagnetic, Immunomagnetic beads, Immunomagnetic separation, Immunoreaction, Immunoreaction procedure, Immunoreaction time, Kgaa, Linear range, Magnetic beads, Negative samples, Newcastle disease virus, Novel electrochemical magnetoimmunosensor, Other viruses, Piranha solution, Planar electrode, Planar gold electrode, Porcine pseudorabies virus, Reproducibility, Same sandwich construction, Same time, Scan rate, Sensitive detection, Signal tags, Simple regeneration step, Single electrode, Target virus, Ultrapure water, Ultrasensitive detection, Verlag, Verlag gmbh, Virus particles, Wang, Weinheim, Weinheim zhang, Zhang, Zhou.
Abstract
A novel electrochemical magnetoimmunosensor for fast and ultrasensitive detection of H9N2 avian influenza virus particles (H9N2 AIV) was designed based on the combination of high‐efficiency immunomagnetic separation, enzyme catalytic amplification, and the biotin–streptavidin system. The reusable, homemade magneto Au electrode (M‐AuE) was designed and used for the direct sensing. Immunocomplex‐coated magnetic beads (IMBs) were easily accumulated on the surface of the M‐AuE to obtain the catalytically reduced electrochemical signal of H2O2 after the immunoreaction. The transducer was regenerated through a simple washing procedure, which made it possible to detect all the samples on a single electrode with higher reproducibility. The magnetic‐bead‐based electrochemical immunosensor showed better analytical performance than the planar‐electrode‐based immunosensor with the same sandwich construction. Amounts as low as 10 pg mL−1 H9N2 AIV could be detected even in samples of chicken dung. This electrochemical magnetoimmunosensor not only provides a simple platform for the detection of the virus with high sensitivity, selectivity, and reproducibility but also shows great potential in the early diagnosis of diseases.
Going viral: A novel electrochemical magnetoimmunosensor for ultrasensitive detection of virus particles is presented. This immunosensor was designed based on the integration of immunomagnetic separation, electrochemical detection, and enzyme catalytic amplification. H9N2 avian influenza virus particles were detected in 1 hour with high sensitivity, selectivity, and reproducibility.
Url:
DOI: 10.1002/asia.201300521
Affiliations:
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type="ISSN">1861-4728</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Anal</term><term>Bioelectron</term><term>Biosens</term><term>Catalytic reaction</term><term>Catalytic signal</term><term>Centrifuge tube</term><term>Chem</term><term>Chicken dung</term><term>Complex samples</term><term>Cyclic voltammograms</term><term>Detection solution</term><term>Different concentrations</term><term>Effective immunomagnetic separation</term><term>Electrochemical</term><term>Electrochemical detection</term><term>Electrochemical immunoassay</term><term>Electrochemical magnetoimmunosensor</term><term>Electrochemical signal</term><term>Electrochemical signals</term><term>Electrode</term><term>Error bars</term><term>First step</term><term>Glass slide</term><term>Glass slides</term><term>Gmbh</term><term>Good reproducibility</term><term>High sensitivity</term><term>Higher reproducibility</term><term>Homemade magneto</term><term>Immunoassay</term><term>Immunological reaction</term><term>Immunomagnetic</term><term>Immunomagnetic beads</term><term>Immunomagnetic separation</term><term>Immunoreaction</term><term>Immunoreaction procedure</term><term>Immunoreaction time</term><term>Kgaa</term><term>Linear range</term><term>Magnetic beads</term><term>Negative samples</term><term>Newcastle disease virus</term><term>Novel electrochemical magnetoimmunosensor</term><term>Other viruses</term><term>Piranha solution</term><term>Planar electrode</term><term>Planar gold electrode</term><term>Porcine pseudorabies virus</term><term>Reproducibility</term><term>Same sandwich construction</term><term>Same time</term><term>Scan rate</term><term>Sensitive detection</term><term>Signal tags</term><term>Simple regeneration step</term><term>Single electrode</term><term>Target virus</term><term>Ultrapure water</term><term>Ultrasensitive detection</term><term>Verlag</term><term>Verlag gmbh</term><term>Virus particles</term><term>Wang</term><term>Weinheim</term><term>Weinheim zhang</term><term>Zhang</term><term>Zhou</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel electrochemical magnetoimmunosensor for fast and ultrasensitive detection of H9N2 avian influenza virus particles (H9N2 AIV) was designed based on the combination of high‐efficiency immunomagnetic separation, enzyme catalytic amplification, and the biotin–streptavidin system. The reusable, homemade magneto Au electrode (M‐AuE) was designed and used for the direct sensing. Immunocomplex‐coated magnetic beads (IMBs) were easily accumulated on the surface of the M‐AuE to obtain the catalytically reduced electrochemical signal of H2O2 after the immunoreaction. The transducer was regenerated through a simple washing procedure, which made it possible to detect all the samples on a single electrode with higher reproducibility. The magnetic‐bead‐based electrochemical immunosensor showed better analytical performance than the planar‐electrode‐based immunosensor with the same sandwich construction. Amounts as low as 10 pg mL−1 H9N2 AIV could be detected even in samples of chicken dung. This electrochemical magnetoimmunosensor not only provides a simple platform for the detection of the virus with high sensitivity, selectivity, and reproducibility but also shows great potential in the early diagnosis of diseases.</div><div type="abstract" xml:lang="en">Going viral: A novel electrochemical magnetoimmunosensor for ultrasensitive detection of virus particles is presented. This immunosensor was designed based on the integration of immunomagnetic separation, electrochemical detection, and enzyme catalytic amplification. H9N2 avian influenza virus particles were detected in 1 hour with high sensitivity, selectivity, and reproducibility.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhou, Chuan Ua" sort="Zhou, Chuan Ua" uniqKey="Zhou C" first="Chuan-Hua" last="Zhou">Chuan-Hua Zhou</name></region><name sortKey="Hong, Zheng Uan" sort="Hong, Zheng Uan" uniqKey="Hong Z" first="Zheng-Yuan" last="Hong">Zheng-Yuan Hong</name><name sortKey="Pang, Dai En" sort="Pang, Dai En" uniqKey="Pang D" first="Dai-Wen" last="Pang">Dai-Wen Pang</name><name sortKey="Shu, Yun" sort="Shu, Yun" uniqKey="Shu Y" first="Yun" last="Shu">Yun Shu</name><name sortKey="Zhang, Zhi Ing" sort="Zhang, Zhi Ing" uniqKey="Zhang Z" first="Zhi-Ling" last="Zhang">Zhi-Ling Zhang</name><name sortKey="Zhang, Zhi Ing" sort="Zhang, Zhi Ing" uniqKey="Zhang Z" first="Zhi-Ling" last="Zhang">Zhi-Ling Zhang</name><name sortKey="Zhang, Zhi Ing" sort="Zhang, Zhi Ing" uniqKey="Zhang Z" first="Zhi-Ling" last="Zhang">Zhi-Ling Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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